Table 3.
Specific activity of the recombinant hCE-1, hCE-2, and human microsomes for pNPA and various Xuorescent substrates
Substrate | hCE-1 | hCE-2 (nmol/min/mg) | Microsomes |
---|---|---|---|
(αR)-A1 | 750 ± 70 | 3000 ± 300 | 65 ± 5 |
(αS)-A1 | 2500 ± 200 | 19,000 ± 100 | 220 ± 10 |
(αR)-A2 | 3300 ± 300 | 14,000 ± 900 | 100 ± 10 |
(αS)-A2 | 16,000 ± 800 | 38,000 ± 1000 | 330 ± 20 |
A3 | 9.5 ± 0.6 | 20 ± 0.3 | 0.24 ± 0.003 |
(2R)-A4 | 0.94 ± 0.03 | 55 ± 1 | 0.087 ± 0.008 |
(2S)-A4 | NDa | ND | ND |
A5 | 0.51 ± 0.01 | 0.56 ± 0.04 | 0.093 ± 0.005 |
A6 | 6.5 ± 0.2 | 4.1 ± 0.1 | 0.10 ± 0.01 |
cis-A7 | 0.51 ± 0.01 | 0.48 ± 0.03 | ND |
trans-A7 | 0.65 ± 0.07 | 1.2 ± 0.02 | ND |
A8 | 4.7 ± 0.3 | 0.71 ± 0.04 | 0.033 ± 0.003 |
pNPA | 18,000 ± 1000 | 43,000 ± 2000 | 1400 ± 100 |
pNPA b | 10,000 | 60,000 |
ND, not detected.
Data are from Senter et al. [27].