Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1976 Sep;31(3):481–494.

Serological relatedness of herpes simplex viruses. Type-specificity of antibody response.

G R Skinner, M E Thouless, S Trueman, J Edwards, A J Gibbs
PMCID: PMC1445242  PMID: 194831

Abstract

The serological relatedness of forty-seven strains of type 1 and type 2 herpes simplex virus was investigated by reciprocal and non-reciprocal neutralization kinetics. Early rabbit antisera divided the virus strains into two distinct groups where confident indentification of virus type was possible. Hyperimmune mouse and rabbit antisera did not divide the two virus types into two distinct non-over-lapping groups. The extent of overlap varied with the particular attribute of the virus being studied. The virus types were best discriminated by their neutralizability by type 1 antisera and least well by their neutralizability by type 2 antisera. The results of reciprocal kinetic neutralization test with hyperimmune mouse antisera were analysed by multi-dimensional cluster analysis. Hyperimmune mouse or rabbit antisera could not be discriminated with respect to their immunogenic type by their absolute neutralization rate constants against either type 1 or type 2 virus, but could be distinguished on a group basis by their relative neutralizability against both virus types (antiserum specificity attribute); however, using this latter criterion, the type of immunogen could only be predicted in seven of the forty antisera under test. 'Early' mouse antisera could also be distinguished as groups by their absolute k-values against type 1 herpes virus. Thus, immunogenic identification, on other than a group basis, was unreliable. The specificity of a given serum was inversely related to its titre. There was a positive correlation between the specificity of a given virus strain and of its corresponding antiserum.

Full text

PDF
481

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARCHETTI I., HORSFALL F. L., Jr Persistent antigenic variation of influenza A viruses after incomplete neutralization in ovo with heterologous immune serum. J Exp Med. 1950 Nov 1;92(5):441–462. doi: 10.1084/jem.92.5.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aurelian L., Royston I., Davis H. J. Antibody to genital herpes simplex virus: association with cervical atypia and carcinoma in situ. J Natl Cancer Inst. 1970 Sep;45(3):455–464. [PubMed] [Google Scholar]
  3. BEDSON S. P., GOSTLING J. V. Further observations on the mode of multiplication of herpes virus. Br J Exp Pathol. 1958 Oct;39(5):502–509. [PMC free article] [PubMed] [Google Scholar]
  4. DUBBS D. R., KIT S. MUTANT STRAINS OF HERPES SIMPLEX DEFICIENT IN THYMIDINE KINASE-INDUCING ACTIVITY. Virology. 1964 Apr;22:493–502. doi: 10.1016/0042-6822(64)90070-4. [DOI] [PubMed] [Google Scholar]
  5. Dowdle W. R., Nahmias A. J., Harwell R. W., Pauls F. P. Association of antigenic type of Herpesvirus hominis with site of viral recovery. J Immunol. 1967 Nov;99(5):974–980. [PubMed] [Google Scholar]
  6. Fazekas de Saint Groth The antigenic subunits of influenza viruses. II. The spectrum of cross reactions. J Immunol. 1969 Nov;103(5):1107–1115. [PubMed] [Google Scholar]
  7. Figueroa M. E., Rawls W. E. Biological markers for differentiation of herpes-virus strains of oral and genital origin. J Gen Virol. 1969 Mar;4(2):259–267. doi: 10.1099/0022-1317-4-2-259. [DOI] [PubMed] [Google Scholar]
  8. Goodheart C. R., Plummer G., Waner J. L. Density difference of DNA of human herpes simplex viruses, types I and II. Virology. 1968 Jul;35(3):473–475. doi: 10.1016/0042-6822(68)90225-0. [DOI] [PubMed] [Google Scholar]
  9. HOGGAN M. D., ROIZMAN B. The isolation and properties of a variant of Herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. Am J Hyg. 1959 Sep;70:208–219. doi: 10.1093/oxfordjournals.aje.a120071. [DOI] [PubMed] [Google Scholar]
  10. Hampar B., Martos L. M., Chakrabarty M., Burroughs M. A. Late 19S rabbit antibody neutralization test for differentiating herpes simplex virus types 1 and 2. J Immunol. 1970 Mar;104(3):593–598. [PubMed] [Google Scholar]
  11. Kieff E. D., Bachenheimer S. L., Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. doi: 10.1128/jvi.8.2.125-132.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
  13. MUNK K., DONNER D. CYTOPATHISCHER EFFEKT UND PLAQUE-MORPHOLOGIE VERSCHIEDENER HERPES-SIMPLEX-VIRUSSTAEMME. Arch Gesamte Virusforsch. 1963 Aug 26;13:529–540. [PubMed] [Google Scholar]
  14. Martin M. L., Palmer E. L., Kissling R. E. Complement-fixing antigens of herpes simplex virus types 1 and 2: reactivity of capsid, envelope, and soluble antigens. Infect Immun. 1972 Feb;5(2):248–254. doi: 10.1128/iai.5.2.248-254.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Nahmias A. J., Josey W. E., Naib Z. M., Luce C. F., Duffey A. Antibodies to Herpesvirus hominis types 1 and 2 in humans. I. Patients with genital herpetic infections. Am J Epidemiol. 1970 Jun;91(6):539–546. doi: 10.1093/oxfordjournals.aje.a121165. [DOI] [PubMed] [Google Scholar]
  16. PLUMMER G. SEROLOGICAL COMPARISON OF THE HERPES VIRUSES. Br J Exp Pathol. 1964 Apr;45:135–141. [PMC free article] [PubMed] [Google Scholar]
  17. Parker J. D., Banatvala J. E. Herpes genitalis; clinical and virological studies. Br J Vener Dis. 1967 Sep;43(3):212–216. doi: 10.1136/sti.43.3.212. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pauls F. P., Dowdle W. R. A serologic study of herpesvirus hominis strains by microneutralization tests. J Immunol. 1967 May;98(5):941–947. [PubMed] [Google Scholar]
  19. Pereira H. G. Influenza: antigenic spectrum. Prog Med Virol. 1969;11:46–79. [PubMed] [Google Scholar]
  20. Plummer G., Goodheart C. R., Miyagi M., Skinner G. R., Thouless M. E., Wildy P. Herpes simplex viruses: discrimination of types and correlation between different characteristics. Virology. 1974 Jul;60(1):206–216. doi: 10.1016/0042-6822(74)90378-x. [DOI] [PubMed] [Google Scholar]
  21. RUSSELL W. C. A sensitive and precise plaque assay for herpes virus. Nature. 1962 Sep 8;195:1028–1029. doi: 10.1038/1951028a0. [DOI] [PubMed] [Google Scholar]
  22. Ratcliffe H. The differentiation of herpes simplex virus type 1 and type 2 by temperature markers. J Gen Virol. 1971 Oct;13(1):181–183. doi: 10.1099/0022-1317-13-1-181. [DOI] [PubMed] [Google Scholar]
  23. Rawls W. E., Iwamoto K., Adam E., Melnick J. L. Measurement of antibodies to herpesvirus types 1 and 2 in human sera. J Immunol. 1970 Mar;104(3):599–606. [PubMed] [Google Scholar]
  24. Rawls W. E., Laurel D., Melnick J. L., Glicksman J. M., Kaufman R. H. A search for viruses in smegma, premalignant and early malignant cervical tissues. The isolation of Herpesviruses with distinct antigenic properties. Am J Epidemiol. 1968 May;87(3):647–655. doi: 10.1093/oxfordjournals.aje.a120855. [DOI] [PubMed] [Google Scholar]
  25. Rawls W. E., Tompkins W. A., Melnick J. L. The association of herpesvirus type 2 and carcinoma of the uterine cervix. Am J Epidemiol. 1969 May;89(5):547–554. doi: 10.1093/oxfordjournals.aje.a120967. [DOI] [PubMed] [Google Scholar]
  26. Roizman B., Keller J. M., Spear P. G., Terni M., Nahmias A., Dowdle W. Variability, structural glycoproteins, and classification of herpes simplex viruses. Nature. 1970 Sep 19;227(5264):1253–1254. doi: 10.1038/2271253a0. [DOI] [PubMed] [Google Scholar]
  27. SCHNEWEIS K. E. [Serological studies on the type differentiation of Herpesvirus hominis]. Z Immun exp ther. 1962 Sep;124:24–48. [PubMed] [Google Scholar]
  28. Sim C., Watson D. H. The role of type specific and cross reacting structural antigens in the neutralization of herpes simplex virus types 1 and 2. J Gen Virol. 1973 May;19(2):217–233. doi: 10.1099/0022-1317-19-2-217. [DOI] [PubMed] [Google Scholar]
  29. Skinner G. R., Taylor J., Edwards J. Precipitating antibodies to herpes simplex virus in human sera: prevalence of antibody to common antigen (band II). Intervirology. 1974;4(5):320–324. doi: 10.1159/000149865. [DOI] [PubMed] [Google Scholar]
  30. Skinner G. R., Thouless M. E., Jordan J. A. Antibodies to type 1 and type 2 herpes virus in women with abnormal cervical cytology. J Obstet Gynaecol Br Commonw. 1971 Nov;78(11):1031–1038. doi: 10.1111/j.1471-0528.1971.tb00221.x. [DOI] [PubMed] [Google Scholar]
  31. Thouless M. E. Serological properties of thymidine kinase produced in cells infected with type 1 or type 2 herpes virus. J Gen Virol. 1972 Dec;17(3):307–315. doi: 10.1099/0022-1317-17-3-307. [DOI] [PubMed] [Google Scholar]
  32. Thouless M. E., Skinner G. R. Differences in the properties of thymidine kinase produced in cells infected with type 1 and type 2 herpes virus. J Gen Virol. 1971 Aug;12(2):195–197. doi: 10.1099/0022-1317-12-2-195. [DOI] [PubMed] [Google Scholar]
  33. WILDY P. Recombination with herpes simplex virus. J Gen Microbiol. 1955 Oct;13(2):346–360. doi: 10.1099/00221287-13-2-346. [DOI] [PubMed] [Google Scholar]
  34. Watson D. H., Shedden W. I., Elliot A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology. 1966 Oct;11(4):399–408. [PMC free article] [PubMed] [Google Scholar]
  35. Watson D. H., Wildy P., Harvey B. A., Shedden W. I. Serological relationships among viruses of the herpes group. J Gen Virol. 1967 Jan;1(1):139–141. doi: 10.1099/0022-1317-1-1-139. [DOI] [PubMed] [Google Scholar]
  36. Wheeler C. E., Jr, Briggaman R. A., Henderson R. R. Discrimination between two strains (types) of Herpes simplex virus by various modifications of the neutralization test. J Immunol. 1969 May;102(5):1179–1192. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES