Abstract
Lymphocytes are heterogeneous with respect to their life-span. Typical B cells, bearing on their membranes immunoglobulin receptors, easily detectable by immunofluorescence, belong mainly to the long-lived population: this can be observed using combined autoradiography and immunofluorescence. However, when primed mice receive (-3H) thymidine before a boosting injection of tobacco mosaic virus (TMV), many plasma cells appearing in the spleen during the secondary response are labelled. In irradiated recipients repopulated with spleen cells from donors primed with TMV and injected with tritiated thymidine 2 hours before killing, the majority of plasms cells appearing in the spleen after antigen injection were labelled. If irradiated mice were repopulated simultaneously with spleen cells from donors primed with TMV and injected with (-3H) thymidine, and from donors primed with haemocyanin, most of the anti-TMV plasms cells were labelled, while most of the anti-haemocyanin plasma cells were unlabelled. These results allowed us to exclude non-specific reutilization of labelled thymidine as the main reason of our observations. It is concluded that either plasma cells derive from shortlived precursors or they receive material from a labelled cell able to co-operate specifically with plasma cell precursors.
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- Askonas B. A., Williamson A. R., Wright B. E. Selection of a single antibody-forming cell clone and its propagation in syngeneic mice. Proc Natl Acad Sci U S A. 1970 Nov;67(3):1398–1403. doi: 10.1073/pnas.67.3.1398. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CRADDOCK C. G., NAKAI G. S., FUKUTA H., VANSLAGER L. M. PROLIFERATIVE ACTIVITY OF THE LYMPHATIC TISSUES OF RATS AS STUDIED WITH TRITIUM-LABELED THYMIDINE. J Exp Med. 1964 Sep 1;120:389–412. doi: 10.1084/jem.120.3.389. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cebra J. J., Goldstein G. Chromatographic purification of tetramethylrhodamine-immune globulin conjugates and their use in the cellular localization of rabbit gamma-globulin polypeptide chains. J Immunol. 1965 Aug;95(2):230–245. [PubMed] [Google Scholar]
- EVERETT N. B., CAFFREY R. W., RIEKE W. O. RECIRCULATION OF LYMPHOCYTES. Ann N Y Acad Sci. 1964 Feb 28;113:887–897. doi: 10.1111/j.1749-6632.1964.tb40710.x. [DOI] [PubMed] [Google Scholar]
- Ellis S. T., Gowans J. L. The role of lymphocytes in antibody formation. V. Transfer of immunological memory to tetanus toxoid: the origin of plasma cells from small lymphocytes, stimulation of memory cells in vitro and the persistence of memory after cell-transfer. Proc R Soc Lond B Biol Sci. 1973 Mar 13;183(1071):125–139. doi: 10.1098/rspb.1973.0009. [DOI] [PubMed] [Google Scholar]
- Gowans J. L. Differentiation of the cells which synthesize the immunoglobulins. Ann Immunol (Paris) 1974 Jan;125C(1-2):201–211. [PubMed] [Google Scholar]
- Harris G. The immune response of spleen explants from primed rabbits to sheep red cells (SRC). I. DNA synthesis in the development of antibody-producing cells (PEC). Immunology. 1973 Feb;24(2):343–363. [PMC free article] [PubMed] [Google Scholar]
- JEENER R. EFFECTS ON THE ANTIGENIC DETERIMINANTS OF TOBACCO MOSAIC VIRUS OF A SMALL NUMBER OF BASE REPLACEMENTS IN THE RIBONUCLEIC ACID. Virology. 1965 May;26:10–15. doi: 10.1016/0042-6822(65)90020-6. [DOI] [PubMed] [Google Scholar]
- Julius M. H., Masuda T., Herzenberg L. A. Demonstration that antigen-binding cells are precursors of antibody-producing cells after purification with a fluorescence-activated cell sorter. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1934–1938. doi: 10.1073/pnas.69.7.1934. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lamelin J. P., Lisowska-Bernstein B., Matter A., Ryser J. E., Vassalli P. Mouse thymus-independent and thymus-derived lymphoid cells. I. Immunofluorescent and functional studies. J Exp Med. 1972 Nov 1;136(5):984–1007. doi: 10.1084/jem.136.5.984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loor F., Forni L., Pernis B. The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins. Eur J Immunol. 1972 Jun;2(3):203–212. doi: 10.1002/eji.1830020304. [DOI] [PubMed] [Google Scholar]
- MITCHELL J., MCDONALD W., NOSSAL G. J. AUTORADIOGRAPHIC STUDIES OF THE IMMUNE RESPONSE. 3. DIFFERENTIAL LYMPHOPOIESIS IN VARIOUS ORGANS. Aust J Exp Biol Med Sci. 1963 Aug;41:SUPPL411–SUPPL421. doi: 10.1038/icb.1963.62. [DOI] [PubMed] [Google Scholar]
- Marbrook J., Haskill J. S. The in vitro response to sheep erythrocytes by mouse spleen cells: segregation of distinct events leading to antibody formation. Cell Immunol. 1974 Jul;13(1):12–21. doi: 10.1016/0008-8749(74)90222-6. [DOI] [PubMed] [Google Scholar]
- Miller J. F. Lymphocyte interactions in antibody responses. Int Rev Cytol. 1972;33:77–130. doi: 10.1016/s0074-7696(08)61449-7. [DOI] [PubMed] [Google Scholar]
- Miller J. J., 3rd, Koskimies S. Studies of nonmigrating, long-lived lymphocytes in rats. I. Reactions after antigenic challenges in situ. Cell Immunol. 1972 Feb;3(2):231–244. doi: 10.1016/0008-8749(72)90162-1. [DOI] [PubMed] [Google Scholar]
- NOSSAL G. J., MAKELA O. Autoradiographic studies on the immune response.I. The kinetics of plasma cell proliferation. J Exp Med. 1962 Jan 1;115:209–230. doi: 10.1084/jem.115.1.209. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pelc S. R., Harris G., Caldwell I. The relationship between antibody formation and deoxyribonucleic acid (DNA) synthesis in mouse spleen during primary and secondary response to sheep erythrocytes (SRC). Immunology. 1972 Aug;23(2):183–197. [PMC free article] [PubMed] [Google Scholar]
- Piguet P. F., Vassalli P. Study of the thymic-derived or -independent nature of mouse spleen cells induced to proliferate in culture by various mitogens and antigens. Eur J Immunol. 1973 Aug;3(8):477–483. doi: 10.1002/eji.1830030805. [DOI] [PubMed] [Google Scholar]
- RIEKE W. O. The in vivo reutilization of lymphocytic and sarcoma DNA by cells growing in the peritoneal cavity. J Cell Biol. 1962 May;13:205–216. doi: 10.1083/jcb.13.2.205. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tannenberg W. J., Malaviya A. N. The life cycle of antibody-forming cells. I. The generation time of 19S hemolytic plaque-forming cells during the primary and secondary responses. J Exp Med. 1968 Nov 1;128(5):895–925. doi: 10.1084/jem.128.5.895. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Urbain-Vansanten G. Concomitant synthesis, in separate cells, of non-reactive immunoglobulins and specific antibodies after immunization with tobacco mosaic virus. Immunology. 1970 Nov;19(5):783–797. [PMC free article] [PubMed] [Google Scholar]
- Urbain J., Van Acker A., De Vos-Cloetens C., Urbain-Vansanten G. Increase and decrease in binding affinity of antibodies during the immune response. Immunochemistry. 1972 Feb;9(2):121–136. doi: 10.1016/0019-2791(72)90033-x. [DOI] [PubMed] [Google Scholar]