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. 2006 May;17(5):2391–2400. doi: 10.1091/mbc.E05-08-0753

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Copyright © 2006, The American Society for Cell Biology

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Figure 6. — Rac3-induced neuritogenesis requires the GTPase activity and the membrane binding of Rac3, whereas it is inhibited by the overexpression of ^HANeurabin I lacking the F-actin or the PDZ domains. (A) FITC-conjugated anti-FLAG immunofluorescence on SK-N-BE cells transfected with ^FlagRac3V12 (V12), ^FlagRac3N17 (N17), ^FlagRac3ΔC-ter (ΔC-ter), or ^FlagRac3 (WT) and analyzed by confocal microscopy. Note that only wild-type ^FlagRac3 stimulates the extension of long and branched neurites. (B) ^FlagRac3 vector (^FlagRac3) was cotransfected in SK-N-BE cells together with the indicated ^HANeurabin I vectors coding for wild type (^HANeurabin I) or F-actin (^HAΔF-actin), PDZ (^HAΔPDZ), or coiled-coil domain (^HAΔ511-721) deletion mutants. The same fields of cells immunostained with FITC-conjugated anti-FLAG (top) and with TRITC-conjugated anti-HA antibodies (bottom) are shown. Note that only wild-type ^HANeurabin I and ^HANeurabin I Δ511-721 allow the ^FlagRac3-induced neuritogenesis. Bar, 10 μm.