Table 1.
Rac3 and Neurabin I interaction in yeast mating experiments
| Yeast Y190a prey | Yeast Y187b bait | 3-ATc (37 mM) | Leu/His/Trpd autotrophy | β-Gale activity |
|---|---|---|---|---|
| pAS2-Rac3 | – | – | – | |
| pACT2 | – | – | – | |
| pACT2 | pAS2-Rac3 | + | – | – |
| pACT2-Neurabin I472-721 | pAS2 | + | – | – |
| pACT2-Neurabin I472-721 | pAS2-Rac3 | + | +++ | +++ |
| pACT2-Neurabin I472-721 | pAS2-Rac3N17 | + | +++ | +++ |
| pACT2-CdGAP | pAS2 | + | – | – |
| pACT2-CdGAP | pAS2-Rac3 | + | ++ | ++ |
| pACT2-CdGAP | pAS2-Rac3N17 | + | + | + |
| pSE 1111f | – | – | – | |
| pSE 1112f | – | – | – | |
| pSE 1111 | pSE 1112 | + | ++ | ++ |
a
The cDNAs of the isolated prey, in frame with the GAL4-AD sequence, were expressed in the Y190 yeast strain
b
The cDNAs of the indicated baits were cloned in frame with the GAL4-BD sequence and expressed in the Y187 yeast strain
c
Molar concentration of 3-amino triazole
d
Colonies grown in selective medium are indicated by + (<50 colonies), ++ (>100), or +++ (>1000)
e
Number of β-galactosidase positive (blue) colonies
f
SNF4 fused to the GAL4-AD (pSE1111) and SNF1 fused to the GAL4-BD (pSE1112) are positive controls of protein–protein interaction