Figure 9.

Model to illustrate EGF-induced interaction between Akt and PLC-γ1. Before EGF stimulation, both PLC-γ1 and Akt are located in the cytosol. The conformation of inactive PLC-γ1 blocks the interaction between PLC-γ1 SH3 domain and the proline-rich motifs of Akt (left). After EGF stimulation, PLC-γ1 translocated to the plasma membrane, and its Y783 was phosphorylated by EGFR, which exposes its SH3 domain for interaction. Simultaneously, Akt was activated by EGFR through PI3K and translocated to the plasma membrane. At the plasma membrane, the PLC-γ1 SH3 domain and Akt proline-rich motifs bind to each other (right).