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. 2006 May;17(5):2415–2423. doi: 10.1091/mbc.E05-11-1037

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Copyright © 2006, The American Society for Cell Biology

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Figure 3. — An origin sequence directs reinitiation (A) The re-replication profile surrounding iYDR309C, a segment that does not re-replicate, in the absence of an ectopic origin is depicted as both the gray histogram and the black dashed line. (B) ARS418, an origin that is associated with a peak on the re-replication profile, directs re-replication at an ectopic locus. The 600-base pair intergenic region containing ARS418 was moved to iYDR309C. Re-replication was induced and the resulting DNA was hybridized to a low-density DNA microarray (gray histogram, Chromosome IV, 800-1400 kb). Superimposed on top is the re-replication profile from the strain without the ectopic ARS418 (black dashed line). (C) An origin with a mutant ACS is not capable of directing re-replication. The essential ACS of ARS418 was mutated and integrated into the re-replicating strain. Re-replication was induced and the resulting DNA hybridized on to a low-density array (gray histogram, Chromosome IV, 800-1400 kb). The re-replication profile of the strain without the ectopic mutant ARS418 is superimposed on top (black dashed line).