Figure 5.

Origins are capable of reinitiating multiple times. (A) Diagram of density transfer experiment. A cartoon depicts what products will look like during the experiment with “heavy” DNA strands shown in black and “light” DNA strands shown in gray. The table briefly describes possible results. (B) ARS428 and ARS418 reinitiate multiple times. DNA from cells that underwent the density transfer protocol described in A was fractionated by CsCl gradient. The resulting fractions were probed for three different classes of DNA sequences as determined by DNA microarray analysis: two origins of reinitiation (ARS418, ▴; ARS428, □), two origins that are re-replicated, but are not sites of reinitiation (ARS1, ▪; ARS1413, ○) and an intergenic sequence that does not re-replicate (iYDR309C, •). The data were normalized by setting the peak of the HL density to a copy number of one.