Table 2.
Strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| YJL310 | leu2-3,112 ura3-52 trp1-289 bar1Δ::LEU2 | Detweiter and Li (1998) |
| YJL3244 | orc2-cdk6A orc6-cdk4A leu2 ura3-52::{pGAL1, URA3} trp1-289 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} | Nguyen et al. (2001) |
| YJL3248 | orc2-cdk6A orc6-cdk4A ura3-52::{pGAL1-Δntcdc6, URA3} trp1-289 leu2 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} | Nguyen et al. (2001) |
| YJL3249 | orc2-cdk6A orc6-cdk4A ura3-52::{pGAL1-Δntcdc6, URA3} trp1-289 leu2 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} | This study |
| YJL4486 | ORC2 ORC6 leu2 ura3-52::{pGAL1, URA3} trp1-289 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} | This study |
| YJL4489 | ORC2 ORC6 ura3-52::{pGAL1-Δntcdc6-cdk2A, URA3} trp1-289 leu2 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} | This study |
| YJL4832 | orc2-cdk6A orc6-cdk4A ura3-52::{pGAL1, URA3} trp1-289 leu2 ade2 ade3 MCM7-2nls3A bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} | This study |
| YJL3240 | orc2-cdk6A orc6-cdk4A ura3-52::{pGAL1-Δntcdc6, URA3} trp1-289 leu2 ade2 ade3 MCM7-2nls3A bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} | This study |
| YJL5038 | his3Δ::KanMX leu2Δ0 met15Δ0 ura3Δ0 bar1Δ::NatMX4 can1Δ::pMFA1-HIS3::pMFα1-LEU2 | This study |
| YJL5493 | orc2-cdk6A orc6-cdk4A leu2 ura3-52::{pGAL1, URA3} trp1-289 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} | This study |
| YJL5834 | ORC2 ORC6 leu2 ura3-52::{pGAL1, URA3} trp1-289 ade2 ade3 MCM7 bar1::LEU2 | This study |
| YJL5787 | ORC2 ORC6 ura3-52::{pGAL1-Δntcdc6-cdk2A, URA3} trp1-289 leu2 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} Δars316::KanMX6 | This study |
| YJL5858 | ORC2 ORC6 ura3-52::{pGAL1-Δntcdc6-cdk2A, URA3} trp1-289 leu2 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} Δars317::KanMX6 | This study |
| YJL5861 | ORC2 ORC6 ura3-52::{pGAL1-Δntcdc6-cdk2A, URA3} trp1-289 leu2 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} Δars318::KanMX4 | This study |
| YJL5816 | ORC2 ORC6 leu2 ura3-52::{pGAL1, URA3} trp1-289 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} cdc7-1 | This study |
| YJL5822 | ORC2 ORC6 ura3-52::{pGAL1-Δntcdc6-cdk2A, URA3} trp1-289 leu2 ade2 ade3 MCM7-2NLS bar1Δ::LEU2 cdc20::{pMET3-HA3-CDC20, TRP1} cdc7-1 | This study |