Table 1.

Quantitation of clusters in control and P1 fibroblasts after BFA treatment

	% of cells with clusters
Golgi protein	CTRL	P1	P1[COG7]
GM130	0	2 ± 1	N.D.
Giantin	3 ± 2	87 ± 1	7 ± 2
GPP130	0	46 ± 4	3 ± 1
GS27	0	0	N.D.
GS28	1 ± 1	38 ± 4	N.D.
βGalT	0	30 ± 7	0

Cells were treated with 0.25 μg/ml BFA for 60 min, and cells with single juxtanuclear clusters within populations of 200–300 were quantitated. Values represent the average of two to three independent experiments. Errors represent SDs. N.D., not determined.