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Mutations in the Ero1p loop can prevent intragenic complementation. (A) Diagram of constructs used in B. ERO1 alleles correspond to products of plasmids pCS75 (1), pCS42 (2), pCS45 (3), pCS341 (4-I66N), pCS342 (4-L70P), pCS321 (4-L70R), pCS318 (4-L84P), pCS319 (4-L86S), and pCS320 (4-W120R). (B) Transformants of CKY598 (ero1-1) containing constructs described in A were plated on SMM (Glu) or SMM Gal (Gal) plates and incubated at 24 or 37°C for 2 d.
