Table 1.
Plasmids
| Name | Description | Markers | Source |
|---|---|---|---|
| pAF82 | ERO1-myc | CEN URA3 | Frand and Kaiser (1998) |
| pAF85 | ERO1-myc | CEN LEU2 | Frand and Kaiser (1998) |
| pAF89 | ERO1-myc | 2μ LEU2 | Frand and Kaiser (1998) |
| pAF125 | pAF89 with C100A | 2μ LEU2 | Frand and Kaiser (1998) |
| pAF126 | pAF89 with C105A | 2μ LEU2 | Frand and Kaiser (1998) |
| pCS214 | pAF89 with C100A, C105A | 2μ LEU2 | This study |
| pAF129 | pAF89 with C352A | 2μ LEU2 | Frand and Kaiser (1998) |
| pAF130 | pAF89 with C355A | 2μ LEU2 | Frand and Kaiser (1998) |
| pCS261 | pAF89 with C352A, C355A | 2μ LEU2 | This study |
| pCS253 | ero1-HA-C90A-C349A-myc with thrombin site (ERO1-t) | 2μ LEU2 | This study |
| pCS257 | pCS253 with C100A | 2μ LEU2 | This study |
| pCS258 | pCS253 with C105A | 2μ LEU2 | This study |
| pCS254 | pCS253 with C352A | 2μ LEU2 | This study |
| pCS255 | pCS253 with C355A | 2μ LEU2 | This study |
| pCS259 | pCS253 with C100A, C105A | 2μ LEU2 | This study |
| pCS256 | pCS253 with C352A, C355A | 2μ LEU2 | This study |
| pCS347 | pCS253 with I66N | 2μ LEU2 | This study |
| pCS349 | pCS253 with L70P | 2μ LEU2 | This study |
| pCS348 | pCS253 with L70R | 2μ LEU2 | This study |
| pCS306 | pCS253 with L84P | 2μ LEU2 | This study |
| pCS307 | pCS253 with L86S | 2μ LEU2 | This study |
| pCS262 | pCS253 with W120R | 2μ LEU2 | This study |
| pCS274 | ero1-C90A-C349A-myc with thrombin site (ERO1-t-myc) | 2μ URA3 | This study |
| pCS275 | ero1-HA-C90A-C349 with thrombin site (ERO1-t-HA) | 2μ LEU2 | This study |
| pCS42 | PGAL-ero1-1-181-myc | CEN LEU2 | This study |
| pCS55 | PGAL ero1-1-181-C100A-C105A-myc | CEN LEU2 | This study |
| pCS341 | pCS42 with I66N | CEN LEU2 | This study |
| pCS342 | pCS42 with L70P | CEN LEU2 | This study |
| pCS321 | pCS42 with L70R | CEN LEU2 | This study |
| pCS318 | pCS42 with L84P | CEN LEU2 | This study |
| pCS319 | pCS42 with L86S | CEN LEU2 | This study |
| pCS320 | pCS42 with W120R | CEN LEU2 | This study |
| pCS75 | ero1-C100A-C105A-HA | CEN URA3 | This study |
| pCS169 | ero1-C100A-C105A-myc | CEN LEU2 | This study |
| pCS196 | pCS169 with I66N | CEN LEU2 | This study |
| pCS194 | pCS169 with L70P | CEN LEU2 | This study |
| pCS235 | pCS169 with L70R | CEN LEU2 | This study |
| pCS233 | pCS169 with L84P | CEN LEU2 | This study |
| pCS230 | pCS169 with L86S | CEN LEU2 | This study |
| pCS184 | pCS169 with W120R | CEN LEU2 | This study |
| pCS343 | pCS261 with I66N | 2μ LEU2 | This study |
| pCS344 | pCS261 with L70P | 2μ LEU2 | This study |
| pCS325 | pCS261 with L70R | 2μ LEU2 | This study |
| pCS322 | pCS261 with L84P | 2μ LEU2 | This study |
| pCS323 | pCS261 with L86S | 2μ LEU2 | This study |
| pCS324 | pCS261 with W120R | 2μ LEU2 | This study |
| ECVS WT | GST-His6-ero1-56-424 (ERO1-c) | AMP | Gross et al. (2004) |
| pCS302 | ECVS WT with C100A-C105A | AMP | This study |
| pCS304 | ECVS WT with L84P-C100A-C105A | AMP | This study |
| pCS305 | ECVS WT with C100A-C105A-W120R | AMP | This study |
| pET Trx1 | Trx1-His6 | KAN | Gross et al. (2005) |