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. 2006 May;17(5):2346–2355. doi: 10.1091/mbc.E05-11-1065

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Copyright © 2006, The American Society for Cell Biology

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Figure 3. — Transient expression of Hsp47 in Hsp47^-/- cells restores normal fibril formation and intracellular localization of type I collagen. (A) Transient Hsp47 expression in Hsp47^-/- cells was analyzed by Western blotting after transfection of Hsp47 expression vector. (B) After transfection of mock vector (control) or Hsp47 expression vector (Hsp47), immunofluorescence staining of type I collagen was carried out without permeabilization. Bar, 50 μm. (C) Hsp47-transfected Hsp47^-/- cells were analyzed by costaining of type I collagen and PDI. (a and b) Type I collagen. (c and d) PDI. (e and f) Merge. (g and h) mDsRed transfection marker. Bar, 20 μm. Note that staining signals were presented by pseudocolors. (D) Hsp47-transfected Hsp47^-/- cells were analyzed by costaining of type I collagen and GM130. Bar, 20 μm. Antibodies used were the same as in Figures 1 and 2.