Figure 5.

APC and EB1 colocalization is a spatiotemporally restricted event. Ax568-N-APC mAb was microinjected into MDCK cells stably expressing EB1-GFP, and total internal reflection fluorescent images were collected 3 h after microinjection (Movie 8). Many EB1 comets lack associated APC, and many APC clusters lack associated EB1; however, a small population of the two structures colocalize (arrow for EB1 and arrowhead for APC) for a short time during the later phases of a MT growth excursion. Bar, 3 μm.