Table 1.
Parameters of MT dynamic instability for APC-decorated and non-APC MTs in MDCK, wild-type, and APC-deficient mouse primary fibroblasts
| MDCK
|
341 (wild-type)
|
335 (APC-null)
|
|||
|---|---|---|---|---|---|
| APC-decorated MTs | Non-APC MTs | APC-decorated MTs | Non-APC MTs | Non-APC MTs | |
| Growth ratea | |||||
| Mean (μm/min) | 4.34 ± 1.91 (164) | 3.88 ± 1.66 (552) | 5.75 ± 4.73 (44) | 5.35 ± 3.12 (400) | 6.08 ± 5.62 (338) |
| Median (μm/min) | 3.83 | 3.36 | 3.83 | 4.21 | 4.89 |
| Shortening ratea | |||||
| Mean (μm/min) | –4.05 ± 2.07 (43) | –6.03 ± 4.58 (415) | –3.73 ± 0.70 (6) | –9.27 ± 7.86 (198) | –7.45 ± 5.62 (222) |
| Median (μm/min) | –3.06 | –4.25 | –3.89 | –6.25 | –5.47 |
| Total events of catastrophe | 32 | 251 | 14 | 134 | 124 |
| Total events of rescue | 30 | 244 | 11 | 121 | 116 |
| Catastrophe frequency (s–1) | 0.0105 | 0.0140 | 0.0092 | 0.0212 | 0.0182 |
| Rescue frequency (s–1) | 0.0098 | 0.0136 | 0.0092 | 0.0191 | 0.0170 |
| Time spent for | |||||
| Growing (%) | 31.4 | 18.1 | 48.0 | 38.2 | 30.5 |
| Shortening (%) | 8.3 | 13.8 | 6.5 | 19.9 | 20.5 |
| Pausing (%) | 60.3 | 68.1 | 45.5 | 41.9 | 50.6 |
| Total time observed (min) | 51.0 | 298.7 | 17.2 | 105.3 | 113.6 |
| No. of cells, microtubules | 10, 36 | 13, 77 | 6, 11 | 7, 29 | 6, 33 |
a
Number of events is given in parentheses