Table 2.

Latrunculin B does not alter motion of tracked granules

	Control ratio of motions	No. of motions	Latrunculin B ratio of motions	No. of motions
R motions	1.05 (0.96–1.15)	3200	1.11 (1.00–1.22)	1800
z motions	1.05 (0.97–1.13)	3200	1.04 (0.93–1.17)	1800

The effects of latrunculin B on the motion of individual, tracked granules labeled with ANP-GFP were determined. Cells were subjected to perfusion with three solutions as follows: 1) intact cells, no digitonin, no latrunculin B (20 s) →; 2) permeabilization, digitonin ± latrunculin B (10 μM) (30 s) →; and 3) no digitonin in the continuing presence or absence of latrunculin B (60 s). The movements of individual granules were tracked for 20 s (2 Hz) before permeabilization (incubation 1) and at the end of incubation 3, and ratios [mean motion in 3 / mean motion in 1] were calculated for each tracked granule. The motions of 80 and 45 granules were tracked in control and latrunculin B-treated cells, respectively. A ratio of greater than one indicates an increase in motion. The numbers in parentheses are the 95% confidence intervals.