Figure 6.

Components of the γ-tubulin complex are not found in large structures around the nuclear envelope or on microtubules in interphase mia1Δ cells. (A) Mto1p-GFP and Alp4-GFP localize to the SPB, several large aggregates around the NE, and along microtubules in interphase wild-type cells. In mia1Δ cells, Mto1p and Alp4p-GFP mainly localize to the SPB. Shown are single maximum intensity reconstructions of z-stacks. (B) Components of the iMTOCs are detected as several distinct dots around the nuclear envelope in wild-type but not mia1Δ cells treated with the microtubule-destabilizing drug MBC. Their SPB localization remains unchanged.