FIG. 5.

Cytoplasmic association of HuR and TIA-1. (A) Polysomal profiles from cells that either were left untreated (Untr.) or were treated with Tn for 2 or 6 h. From left to right, fractions lacked ribosomes or ribosome subunits (fractions 1 and 2), contained ribosome subunits or single ribosomes (fractions 3 to 5), or spanned polysomes of increasing molecular weights (fractions 6 to 10). The relative abundance levels of 28S and 18S rRNAs were visualized from ethidium bromide-stained agarose gels. (B) Protein from each fraction was prepared for Western blot analysis to detect HuR and TIA-1.