FIG. 6.

Association of HuR and TIA proteins. (A) IP assays were carried out using cytoplasmic lysates from HeLa cells that were either left untreated or were treated with Tn for the indicated times using an anti-TIA-1 antibody. The presence of TIA-1, HuR, and TIAR in the IP material was monitored by Western blotting. (B) IP reactions were performed in the absence (−) or presence (+) of RNase A and RNase T1, using cytoplasmic lysates and either IgG or anti-TIA-1 antibody. The presence of TIAR and HuR in the IP material was monitored by Western blotting (WB). H.C., heavy immunoglobulin chain.