FIG. 1.

Expression of the inducible transgene coding for the super-repressor and GFP in tTA/IκBα-AA double transgenic mice is restricted to neurons. (A) Schematic diagram of the binary inducible transgenic system used to inhibit NF-κB activity in the forebrain. The internal ribosome entry site has been mutated to render GFP a real tracer of super-repressor (IκBα-AA) expression. In the absence of DOX, an analog of tetracycline that passes the blood-brain barrier, super-repressor and GFP are expressed in a polycistronic transcript. SV40, simian virus 40; HPRT, hypoxanthine phosphoribosyltransferase; prom, promoter. (B) Coronal sections from the brain of an 8-week-old tTA/IκBα-AA double transgenic subjected to immunohistochemical analysis with antibodies against GFP and NeuN or GFAP and photographed in the CA1 region. Green, GFP immunoreactivity; red, NeuN immunoreactivity or GFAP immunoreactivity; yellow, double-labeled cells; white arrowheads, neuronal cells; white arrows, glial cells; scale bar, 20 μm.