FIG. 3.

Nucleocytoplasmic shuttling of Pak5. (A) CHO control cells and stable cell lines expressing Myc-tagged Pak5 were left untreated or were treated for 12 h with LMB. Nuclear extraction was performed, and equal amounts of lysates were subjected to SDS-PAGE and revealed with anti-Myc antibodies. Anti-PCNA antibodies were used as a qualitative and quantitative control. (B) Stable CHO cell lines expressing GFP or GFP-Pak5 were treated for 12 h with LMB, fixed, and analyzed by fluorescence microscopy. Bar = 20 μm. (C) Sequence alignment of the functional Pak5 NES, as defined by deletion studies, and known NES elements in representative proteins.