FIG. 4.

Molecular analysis of the thyroid hormone responsive element present in the catnb gene. (A) Structure of the four putative DR4 found in intron 1. Divergences from canonical DR4 are indicated in boldface type. (B) WT TREint-1, showing the GT sequences (1 to 6) substituted in the different TRE mutants. (C to E) EMSA analysis using the labeled probes indicated and in vitro-transcribed/translated proteins indicated. The specificity of the binding was assessed by adding specific or nonrelated cold sequences at the indicated molar excess (Comp. 10× or Comp. 100×). +, present; −, absent.