FIG. 10.
The histone deacetylase inhibitor TSA and the protein methylation inhibitor d-MTA increase SP-A expression in lung type II cells. Human fetal lung type II cells were cultured in medium in the presence of DMSO vehicle or with TSA (50 nM) or d-MTA (300 μM) dissolved in DMSO for 24 h in a 20% O2 environment followed by coincubation in the absence (control) or presence of Bt2cAMP for an additional 24 h. Cytoplasmic fractions were analyzed for SP-A by immunoblotting. Shown is a representative immunoblot of this experiment, which was repeated three times with comparable results.