FIG. 5.

Cyclic AMP- and IL-1-induced recruitment of CBP and SRC-1 to the TBE is inhibited by hypoxia. Type II cells were incubated for 24 h in control medium in a 2% or 20% O₂-containing environment and then incubated for another 6 or 24 h with cAMP or IL-1, alone or in combination. The soluble cross-linked chromatin was immunoprecipitated using specific antibodies to CBP and SRC-1. After immunoprecipitation, DNA was analyzed for the 75-bp region containing the TBE (Fig. 1A) using real-time PCR. The data are expressed as arbitrary units. Shown is a representative of four independent experiments. The bars represent the means ± SEM of values from triplicate dishes of type II cells.