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The nuclear import of Rpf1 is impaired in kap120 kap114 nmd5 triple mutants. Wild-type yeasts, cells with NMD5, KAP114, or KAP120 deleted, and strains carrying the indicated double or triple mutations were cultured in liquid-rich media at 30°C. The cells, which all contain a chromosomal integration of RPF1-GFP, were examined by immunofluorescence microscopy. The samples were probed with GFP-specific antibodies and with FITC-conjugated secondary antibodies and stained with DAPI.
