FIG. 7.

RPF1 overexpression in kap120 mutants relieves the export defect of large ribosomal subunits. (A) Wild-type cells, deletion mutants for NMD5 or KAP114, and KAP120-deletion mutants transformed with a multicopy plasmid (2μm) or with a 2μmRPF1 plasmid were grown in liquid media at 25°C. The cells, which all contain a chromosomal L11-GFP allele, were examined by immunofluorescence microscopy. The samples were probed with anti-GFP antibodies and with FITC-conjugated secondary antibodies, and the DNA was stained with DAPI. (B) The cells shown in the lower two sections of panel A were grown in liquid media and analyzed by SDS-PAGE and Western blotting with affinity-purified Rpf1-specific antibodies.