TABLE 1.
Yeast strains and plasmids used in this study
| Strain or plasmid description | Genotype or encoded protein | Source or reference |
|---|---|---|
| Strains | ||
| Wild type | MATaura3-52 leu2Δ1 his3Δ200 trp1Δ63 (S288C, GSY155) | 51 |
| EGY48 | MATα ura3 leu2 his3 trp1 lexA op::LEU2 (GSY168) | OriGene |
| RPF1-GFP | MATaura3-52 leu2Δ1 his3Δ200 trp1Δ63 RPF1-GFP::HIS3 (GSY1044) | This study |
| prp20-1 RPF1-GFP | MATα ura3 leu2 trp1 prp20-1 RPF1-GFP::TRP1 (GSY1052) | This study |
| Δkap120 RPF1-GFP | MATα ura3 leu2 his3 trp1 KAP120::TRP1 RPF1-GFP::HIS3 (GSY1045) | This study |
| Δkap114 RPF1-GFP | MATα ura3 leu2 his3 trp1 KAP114::LEU2 RPF1-GFP::HIS3 (GSY1046) | This study |
| Δnmd5 RPF1-GFP | MATα ura3 leu2 his3 trp1 NMD5::TRP1 RPF1-GFP::HIS3 (GSY1050) | This study |
| Δkap114 Δkap120 RPF1-GFP | MATα ura3 leu2 his3 trp1 KAP114::LEU2 KAP120::TRP1 RPF1-GFP::HIS3 (GSY1047) | This study |
| Δnmd5 Δkap120 RPF1-GFP | MATα ura3 leu2 his3 trp1 NMD5::TRP1 KAP120::URA3 RPF1-GFP::HIS3 (GSY1051) | This study |
| Δnmd5 Δkap114 RPF1-GFP | MATα ura3 leu2 his3 trp1 NMD5::TRP1 KAP114::LEU2 RPF1-GFP::HIS3 (GSY1049) | This study |
| Δnmd5 Δkap114 Δkap120 RPF1-GFP | MATα ura3 leu2 his3 trp1 NMD5::TRP1 KAP114::LEU2 KAP120::URA3 RPF1-GFP::HIS3 (GSY1048) | This study |
| RPL11b-GFP | MATaura3 leu2 his3 trp1 RPL11b-GFP::HIS3 (GSY1036) | This study |
| Δnmd5 RPL11b-GFP | MATα ura3 leu2 his3 trp1 NMD5::TRP1 RPL11b-GFP::HIS3 (GSY1043) | This study |
| Δkap114 RPL11b-GFP | MATα ura3 leu2 his3 trp1 KAP114::LEU2 RPL11b-GFP::HIS3 (GSY1042) | This study |
| Δkap120 RPL11b-GFP | MATα ura3 leu2 his3 trp1 KAP120::TRP1 RPL11b-GFP::HIS3 (GSY1039) | This study |
| rpf1-1 | MATaura3 leu2 his3 trp1 ade2 RPF1::HIS3 (pRS315-rpf1-1) (GSY1076) | This study |
| rpf1-1 RPL11b-GFP | MATaura3 leu2 his3 trp1 ade2 RPF1::HIS3 RPL11b-GFP::TRP1 (pRS315-rpf1-1) (GSY1148) | This study |
| Plasmids | ||
| pSH18-34 (pGS479) 2μm URA3 lexA op::lacZ | β-Galactosidase | OriGene |
| pEG202 (pGS474) 2μm HIS3 | lexA DNA BD | OriGene |
| pEG-Bam (pGS898) 2μm HIS3 | lexA DNA BD | This study |
| pEG-KAP120 (pGS1020) | BD-Kap120 | This study |
| pJG4-5 (pGS476) 2μm TRP1 | B42 AD | OriGene |
| pJG-Bam (pGS898) 2μm TRP1 | B42 AD | This study |
| pJG-GSP1 (pGS893) | AD-Gsp1 | This study |
| pJG4-5-RPF1 47-295 (pGS1600) | AD-Rpf1 (47-295, library isolate) | This study |
| pJG-RPF1 (pGS1460) | AD-Rpf1 (1-295, full-length) | This study |
| pQE9-GSP1 Q71L (pGS468) | 6His-Gsp1 Q71L | 35 |
| pQE60-NMD5 (pGS1090) | Nmd5-6His | 18 |
| pQE60-KAP120 (pGS1441) | Kap120-6His | This study |
| pQE30-RPF1 (pGS1465) | 6His-Rpf1 | This study |
| pGEX-5G-KAP120 (pGS1235) | GST-Kap120 | This study |
| pGEX-4T-SXM1 (pGS1295) | GST-Sxm1 | This study |
| pGEX-4TEV-KAP104 (pGS1010) | GST-Kap104 | 35 |
| pGEX-4TEV-MTR10 (pGS882) | GST-Mtr10 | This study |
| pGEX-4T-KAP114 (pGS1188) | GST-Kap114 | This study |
| pGEX-4T-NMD5 (pGS1104) | GST-Nmd5 | This study |
| pGEX-4TEV-KAP95 (pGS962) | GST-Kap95 | 35 |
| pGEX-4T-PDR6 (pGS1124) | GST-Pdr6 | This study |
| pGEX-4T-MSN5 (pGS1095) | GST-Msn5 | This study |
| YEpGAL-GST-SXM1 (pGS1209) 2μm URA3 | GST-Sxm1 | 18 |
| YEpGAL-GST-PSE1 (pGS338) 2μm URA3 | GST-Pse1 | 52 |
| YEpGAL-GST-YRB4 (pGS334) 2μm URA3 | GST-Yrb4 | 52 |
| YEpGAL-GST-RPF1 (pGS1466) 2μm URA3 | GST-Rpf1 | This study |
| YCpGAL-GFP-KAP120 (pGS1233) CEN URA3 | GFP-Kap120 | This study |
| YCpGAL-KAP120 (pGS1231) CEN URA3 | Kap120 | This study |
| YCpGAL-KAP120ΔN CEN URA3 | Kap120ΔN (133-1,032) | This study |
| YCpGAL-SXM1 (pGS1206) CEN URA3 | Sxm1 | This study |
| YCpGAL-SXM1ΔN (pGS1210) CEN URA3 | Sxm1ΔN (125-944) | This study |
| pQE70-T7-RPF1 (pGS1298) | Rpf1 | This study |
| pRS316-RPF1 (pGS1454) CEN URA3 | Rpf1 | This study |
| pRS315-RPF1 (pGS1453) CEN LEU2 | Rpf1 | This study |
| pRS315-rpf1-1 (pGS1601) CEN LEU2 | Rpf1-1 | This study |
| pRS426-RPF1 (pGS1455) 2μm URA3 | Rpf1 | This study |