FIG. 3.
Ribozyme-mediated self-cleavage of nascent mRNA results in a partial suppression of replication slowdown. (A) Scheme of the region containing the ribozyme sequence. The 346-bp ribozyme is depicted in the form of a secondary-structure RNA sequence placed at the 3′ end of the lacZ transcript. (B) Northern blot analysis of full-length lacZ mRNA transcripts. (C) 2D gel analysis of replication intermediates of pRWY012 constructs isolated from hpr1Δ cells. Hybridization against a 3.2-kb SmaI-SacI fragment is shown. Schemes of the construct containing the inactive (ribm) or active (Rib+) ribozyme sequence are indicated on the top. Distribution and quantification of replication intermediates within the descending simple-Y arc are presented at the bottom. For other details, see the legend to Fig. 2.