TABLE 2.
RubisCOa activity in crude extracts of cells grown on 10 mM and 25 mM NaHCO3
| Medium and strain | pH 7.2 | pH 8.0 |
|---|---|---|
| 10 mM NaHCO3 | ||
| CGA010 (wild type) | 141 ± 12 | 175 ± 28 |
| CGA2024 (ΔcbbRRS) | 143 ± 13 | 154 ± 26 |
| CGA2025 (ΔcbbR) | 32 ± 24 | 27 ± 18 |
| CGA2034 (ΔcbbSR) | 81 ± 3 | 114 ± 6 |
| CGA2083 (ΔcbbRR2) | 75 ± 6 | 93 ± 5 |
| CGA2028 (ΔcbbLS) | 30 ± 8 | 36 ± 12 |
| CGA2071 (ΔcbbM) | 50 ± 11 | 76 ± 19 |
| CGA2063 (ΔcbbRR1, ΔcbbRR2) | 78 ± 31 | 89 ± 30 |
| CGA2064 (ΔcbbR, ΔcbbRRS) | 54 ± 24 | 66 ± 27 |
| CGA2091 (ΔcbbRR1, ΔcbbRR2, ΔcbbM) | 12 ± 5 | 18 ± 7 |
| CGA2098 (ΔcbbSR, ΔcbbM) | 31 ± 5 | 46 ± 4 |
| 25 mM NaHCO3 | ||
| CGA010 (wild type) | 34 ± 8 | 41 ± 9 |
| CGA2024 (ΔcbbRRS) | 48 ± 20 | 50 ± 17 |
| CGA2025 (ΔcbbR) | 12 ± 4 | 16 ± 6 |
| CGA2034 (ΔcbbSR) | 26 ± 11 | 32 ± 13 |
| CGA2083 (ΔcbbRR2) | 31 ± 4 | 32 ± 4 |
| CGA2028 (ΔcbbLS) | 9 ± 5 | 8 ± 4 |
| CGA2071 (ΔcbbM) | 25 ± 8 | 35 ± 11 |
| CGA2063 (ΔcbbRR1, ΔcbbRR2) | 49 ± 7 | 55 ± 11 |
| CGA2064 (ΔcbbR, ΔcbbRRS) | 21 ± 6 | 21 ± 8 |
| CGA2091 (ΔcbbRR1, ΔcbbRR2, ΔcbbM) | 14 ± 2 | 23 ± 2 |
| CGA2098 (ΔcbbSR, ΔcbbM) | 11 ± 2 | 16 ± 3 |
a
Activities are expressed as nanomoles of CO2 fixed per minute per milligram. Each value is the average of at least four independent experiments repeated in duplicate.