TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| C. glutamicum | ||
| ATCC 13032 | Biotin-auxotrophic wild type | 22 |
| 13032ΔdtxR | In-frame deletion of the dtxR gene | This work |
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Invitrogen |
| BL21(DE3) | ompT hsdSB(rB−mB−) gal dcm (DE3) | 35 |
| Plasmids | ||
| pK19mobsacB | Kanr; vector for allelic exchange in C. glutamicum; (pK18 oriVE.c.sacB lacZα) | 32 |
| pK19mobsacB-ΔdtxR | Kmr; pK19mobsacB derivative containing a crossover PCR product covering the up- and downstream regions of dtxR | This work |
| pET24b | Kanr; vector for overexpression of genes in E. coli, adding a C-terminal hexahistidine affinity tag to the synthesized protein (pBR322 oriVE.c.PT7lacI) | Novagen |
| pET24b-dtxR-C | Kanr; pET24b derivative for overproduction of DtxR with a C-terminal decahistidine tag—the four additional histidines were attached to the dtxR fragment | 40 |