TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Reference |
|---|---|---|
| B. methanolicus | ||
| MGA3 | Wild-type strain | 26 |
| MGA3C-A6 | MGA3 cured of natural plasmid pBM19 | 8 |
| E. coli | ||
| DH5α | General cloning host | BRL |
| Plasmids | ||
| pGEM-11zf | E. coli cloning vector; Apr | Promega |
| pTB1.9 | E. coli-B. subtilis shuttle vector; Apr Neor | 8 |
| pTB1.9mdhL | pTB1.9 carrying the mdh gene | 8 |
| pHP13 | E. coli-B. subtilis shuttle vector; Clmr | 14 |
| pHPhps+phib | pHP13 harboring hps and phi genes | This study |
a
Apr, ampicillin resistance; Neor, neomycin resistance; Clmr, chloramphenicol resistance.
b
This plasmid is described in Materials and Methods.