TABLE 2.
Deoxyoligonucleotide primers used for rt-PCR
| Primer name | Sequence (5′-3′) | Orientation and position in target |
|---|---|---|
| pBM19-primers | ||
| rpe-CF | CGGAGCCGATTACATTCA | Sense, 4432 in pBM19 |
| rpe-CR | GCCAGGATTAACCGTCAT | Antisense, 4778 in pBM19 |
| pfk-CF | AAGTGCCATCTCCACCAATC | Sense, 8142 in pBM19 |
| pfk-CR | CCAGGAATGAACGCTGCTAT | Antisense, 8419 in pBM19 |
| tkt-TR | AACGACCGCACGAATAGC | Sense, 8386 in pBM19 |
| tkt-TF | ATGGCCTGTGCCAATTCT | Antisense, 8916 in pBM19 |
| tkt-CF | CGATATCATTGCCGTCCTCA | Sense, 10436 in pBM19 |
| tkt-CR | ATGCCACAACAGGTCCGTTA | Antisense, 10772 in pBM19 |
| fbaT-rt1 | GGATGGCCTGATCCAACCTT | Sense, 11020 in pBM19 |
| fbaT-rt2 | CTCCACGGTGGTACAGGTAT | Antisense, 11382 in pBM19 |
| fba-RTB | CTTCTTGTCCACCGATACGA | Sense, 11564 in pBM19 |
| fba-RTA | CGTAACGGTACCAGTTGCTA | Antisense, 11776 in pBM19 |
| glpXT-R | CTGTCCAACAGCATATCC | Sense, 11934 in pBM19 |
| glpXT-F | GTCGCAGTAACGATTAGC | Antisense, 12476 in pBM19 |
| glpX-RTA | GTTCCGAGTTCCTCTCCAAT | Sense, 13311 in pBM19 |
| glpX-RTB | AAGCGTGTTCAGTCGTTAGC | Antisense, 13535 in pBM19 |
| mdhT-RT | ACGGTACGAGACATCATTCC | Sense, 13832 in pBM19 |
| mdhT-RT | CACGCGTTCCTACTGTTCAA | Antisense, 14156 in pBM19 |
| mdh-RTA | GCTGTATCGTGAGAGCTACC | Sense, 14944 in pBM19 |
| mdh-RTB | TCCACCAGCCAGCGTAATTG | Antisense, 15232 in pBM19 |
| parA-rt1 | TCTTCGGCACTGTTGAAGGA | Sense, 16916 in pBM19 |
| parA-rt2 | TCCAGCCTGAAGGATATAGC | Antisense, 17080 in pBM19 |
| repB-rt1 | GTCTGTTCGTCCTTGGACTT | Sense, 18220 in pBM19 |
| repB-rt2 | AATGCTTGGAGCCGAGGATA | Antisense, 18587 in pBM19 |
| Chromosomal primers | ||
| hps-RTA | GTAGCTGAGGTTCAGGAGTA | Sense, 54 in hps |
| hps-RTB | CTGCGATCATGTCAACAAGG | Antisense, 336 in hps |
| hpsT-rtF | TCATTGTTGGCGGCGGTATT | Sense, 549 in hps |
| hpsT-rtR | AGTGCTTCGGCTTCTTCATC | Antisense, 85 in phi |
| phi-rt1 | GAAGAAGCCGAAGCACTGGT | Sense, 68 in phi |
| phi-rt2 | TGATCGTTACAGCCGCGATG | Antisense, 339 in phi |
| act-RTA | CCTGGTGCTGTAGCTGTAAT | Sense, 128 in act |
| act-RTB | GCTTCGTCAAGTGTCAGTTC | Antisense, 463 in act |
| rRNA-1F | AAGATGGCTTCGGCTATCAC | Sense, 179 in 16SrRNA |
| rRNA-1R | CGAACGGTACTTGTTCTTCC | Antisense, 443 in 16SrRNA |