TABLE 1.

Identities of proteins in 2DE spots showing statistically significant differences in expression levels in lysates from rpoS mutant and wild-type cells

Spot no.	Abundance (rpoS/ wild-type ratio)a	No. of peptidesc	Locus identification	Mol wtd	pIe	Common namef	Main role
293	0.29	6	GSU2435	44,327	4.9	Dehydrogenase complex E2-component, dihydrolipamide acetyltransferase	Energy metabolism
339	0.07	20	GSU0674	59,674	6.4	Prismane protein	Energy metabolism
386	NDb	45	GSU0674	59,674	6.4	Prismane protein	Energy metabolism
498	0.2	55	GSU3294	45,460	6	Rubredoxin-oxygen oxidoreductase, putative	Energy metabolism
647	0.47	9	GSU1921	27,701	5.8	Ribosomal protein S2 (rpsB)	Protein synthesis
714	0.27	3	GSU2441	22,778	4.8	Conserved hypothetical protein	Hypothetical protein
728	0.07	21	GSU0217	20,565	5.2	Nitroreductase family proteins	Unknown function
737	ND	8	GSU0804	21,480	6.5	Trp repressor-binding protein WrbA	Energy metabolism
768	ND	36	GSU3289	19,004	5.3	Conserved hypothetical protein	Hypothetical protein
769	ND	22	GSU1642	19,061	5	Conserved hypothetical protein	Hypothetical protein
1159	ND	32	GSU1394	139,563	5	Laccase family protein	Unknown function
1200	0.14	45	GSU1158	21,639	6.1	Superoxide dismutase (sodA)	Cellular processes
134	2.5	30	GSU0658	96,456	5.6	ATP-dependent Clp protease, ATP-binding subunit ClpB	Protein fate
404	2.1	19	GSU0332	52,228	6	Aminopeptidase A/I (pepA)	Protein fate
719	2.4	2	GSU3096	23,229	4.9	Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase	Amino acid biosynthesis
1167	2.1	54	GSU0580	97,111	5.6	Pyruvate phosphate dikinase (ppdK)	Energy metabolism

^aRatio of the average integrated densities (spot volumes) of protein spots displaying quantitative differences in integrated density with a P value of at least 0.05 after statistical analysis using a two-tailed Student t test.

^bND, not detected

^cNumber of tryptic peptides with masses that matched, within the acceptance criteria (DeltaCN and Xcorr) specified in Materials and Methods, the open reading frame (locus identification).

^dPredicted protein molecular weight based on the corresponding open reading frame sequence.

^ePredicted isoelectric point based on the corresponding open reading frame sequence.

^fAnnotation from http://www.ncbi.nlm.nih.gov/.