FIG. 2.
Oxygen consumption by recombinant D. gigas ROO. (A) Representative oxygen consumption assay by D. gigas ROO monitored with a Clark-type electrode. FlRd reductase, E. coli flavorubredoxin reductase; Dg_Rd, D. gigas recombinant rubredoxin. (B) Hyperbolic dependence of the oxygen reductase activity of ROO on the concentration of rubredoxin. Inset, linear dependence of the oxygen reductase activity of ROO on the concentration of E. coli FlRd reductase (tested range, 100 to 800 nM) at a fixed rubredoxin concentration of 16 μM.