FIG. 3.

RT-PCR analysis of Wβ phage gene expression during the lysogenic state. RNA prepared from cultures grown under three different conditions was reverse transcribed with random primers. Subsequent PCR was performed with primers specific for the indicated Wβ phage genes. (A) Analysis with RNA from overnight BHI broth cultures of two different B. anthracis (ΔSterne::φWβ) lysogens (+) and the parental nonlysogen (−) and from the B. cereus (RSVF1::φWβ) lysogen (+) and nonlysogen (−). (B) Analysis of RNA isolated from only B. anthracis and B. cereus Wβ lysogens grown for 5 h in either liquid sporulation medium (+) or BHI medium (−).