FIG. 1.

Membrane binding of MinD and cytochrome b₅. (A) The MTS of MinD, shown as a cylinder, is an amphipathic α helix oriented parallel to the phospholipid bilayer. Nonpolar amino acid side chains extend downward into the interior of the bilayer. (B) The hydrophobic MBD of cytochrome b₅ is deeply inserted in the phospholipid bilayer. (C) Schematic representation of AD-Yfp-MinD (top) and AD-Yfp-MinDΔ10-b5MBD (bottom). The positions of C-terminal MTS of MinD, the b5MBD, and the NLS are indicated.