FIG. 4.
Targeting of MinC to nuclear membrane and cell division phenotype of ΔminDE strains. (A through D) Yeast AH109 cells expressed the AD fusion protein shown together with BD-MinC-MTSEc (A) or BD-MinC-MTSBs (C). The yeast nuclear membrane (NM) is shown by the arrows. (A and C) YFP image; (B and D) DIC image. (E through H) HL1 E. coli cells (minC+ ΔminDE) expressed the plasmid-encoded proteins shown at the left. (E and G) Cells were grown in presence of 0.4% glucose to suppress expression of the plasmid-encoded proteins. (F and H) Cells were grown in presence of 10 μM IPTG to induce expression of the plasmid-encoded proteins. minC was expressed constitutively from the chromosome under control of the normal min promoter.