TABLE 2.
MinD interactions in the yeast two-hybrid systema
| GAL4 AD fused to: | GAL4 BD fused to:
|
|||
|---|---|---|---|---|
| MinD | MinDΔ10 | MinC | MinE1-31 | |
| MinD | 100 ± 3.5 | 0.74 ± 0.16 | 100 ± 1.4 | 100 ± 3.1 |
| MinDΔ10 | 0.38 ± 0.06 | 4.1 ± 0.24 | 49 ± 4 | 41 ± 0.2 |
| MinC | 12.8 ± 1 | 113.2 ± 23 | ND | ND |
| Nothing | 0.4 ± 0.08 | 0.55 ± 0.11 | 0.09 ± 0.02 | 0.6 ± 0 |
a
Interaction is indicated as β-galactosidase activity; relative activity is shown as the percentage of MinD-MinD interaction (190 Miller units) (for MinD and MinDΔ10 columns), MinD-MinC interaction (1,453 Miller units) (for the MinC column), and MinD-MinE1-31 interaction (250 Miller units) (for the MinE1-31 column). The interactions were further confirmed by the ability of AH109 cotransformed with each of the paired constructs to grow in the absence of histidine and adenine. All cotransformants grew in the absence of histidine and adenine except for the six pairs with β-galactosidase relative activity less than 0.75. ND, not determined.