TABLE 3.
Interactions of MinDΔ10-b5MBD in the yeast two-hybrid system
| GAL4 AD fused to: | GAL4 BD fused to: | Activitya |
|---|---|---|
| Yfp-MinD | MinD | 100 ± 6 |
| Yfp-MinDΔ10 | MinD | 0.8 ± 0.1 |
| Yfp-MinDΔ10-b5MBD | MinDΔ10-b5MBD | 58 ± 2.2 |
| Yfp-MinDΔ10 | MinDΔ10-b5MBD | 1 ± 0.8 |
| Yfp-MinDΔ10-b5MBD | MinDΔ10 | 2 ± 0.4 |
| Yfp-MinD | MinDΔ10-b5MBD | 8 ± 0.7 |
| YFP-MinDΔ10-b5MBD | MinD | 5 ± 0.5 |
| b5MBD | MinDΔ10-b5MBD | 1 ± 0.2 |
| Yfp-MinDΔ10-b5MBD | b5MBD | 1 ± 0.4 |
| Yfp-MinD | MTS | 0.6 ± 0.2 |
| MTS | MinD | 0.8 ± 0.2 |
| Yfp-MinDΔ10 | MinDΔ10 | 3.9 ± 0.6 |
| Yfp-MinD | MinC | 100 ± 15 |
| Yfp-MinDΔ10-b5MBD | MinC | 111.6 ± 3.8 |
| Yfp-MinD | MinE1-31 | 100 ± 16 |
| Yfp-MinDΔ10-b5MBD | MinE1-31 | 165.4 ± 8.3 |
| Nothing | MinD | 1 ± 0.2 |
a
β-Galactosidase activity is normalized to interactions between Yfp-MinD and MinD (67 Miller units) (for rows 1 through 12 and 17), Yfp-MinD and MinC (1,117 Miller units) (for rows 13 and 14), and Yfp-MinD and MinE1-31 (34 Miller units) (for rows 15 and 16). The interactions were further confirmed by the ability of AH109 cotransformants to grow in the absence of histidine and adenine; growth was observed only in the strains indicated on rows 1, 3, 6, 7, and 12 through 16.