FIG. 2.

HPLC separations of [ring-¹⁴C₆]-benzoyl-CoA and products resulting from its conversion with purified Box enzymes. Benzoyl-CoA (A), 2,3-dihydro-2,3-dihydroxybenzoyl-CoA (dihydrodiol) resulting from transformation of benzoyl-CoA with the oxygenase/reductase (BoxBA) (B), and 3,4-dehydroadipyl-CoA semialdehyde and formate (C) are the products resulting from the hydrolytic ring opening of the dihydrodiol as catalyzed by BoxC_mal. (D) Transformation of the semialdehyde to the corresponding acid 3,4-dehydroadipyl-CoA is catalyzed by BoxD_mal. The small amount of benzoate results from the thioesterase action on benzoyl-CoA.