FIG. 6.

Effect of esc mutations on secretion of the Esp proteins and Tir effector. (A) Secreted-protein profiles of WT EPEC and various mutant strains. Bacteria were grown in DMEM, and the secreted proteins were resolved by 12% SDS-PAGE and stained with CBB. (B) Immunoblot analysis of Tir and EspA in the culture supernatants (Sup.) and whole-cell extracts (Cell). Anti-EspA and -Tir antibodies were used for immunodetection. Lanes M and WT indicate the size markers and secreted proteins prepared from the EPEC wild type, respectively.