TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| pABB-CRS2 | Positive-selection suicide vector | 31 |
| pDONR201 | Gateway cloning vector | Invitrogen |
| pDONR-escC | escC cloned into pDONR201 | This study |
| pDONR-escD | escD cloned into pDONR201 | This study |
| pDONR-escJ | escJ cloned into pDONR201 | This study |
| pDONR-GST-escD | escD cloned into pDONR201 | This study |
| pDONR-GST-escF | escF cloned into pDONR201 | This study |
| pDONR-escD-V5 | escD cloned into pDONR201 | This study |
| pDONR-escF-V5 | escF cloned into pDONR201 | This study |
| pABB-ΔescC | escC deletion in pABB-CRS2 | This study |
| pABB-ΔescD | escD deletion in pABB-CRS2 | This study |
| pABB-ΔescJ | escJ deletion in pABB-CRS2 | This study |
| pTrc99A | Prokaryotic expression vector | Pharmacia |
| pABB-Trc99cm | pTrc99A-derived vector | 31 |
| p99-escC | escC cloned into pABB-Trc99cm | This study |
| p99-escD | escD cloned into pABB-Trc99cm | This study |
| p99-escJ | escJ cloned into pABB-Trc99cm | This study |
| p99-espA | espA cloned into pABB-Trc99cm | 31 |
| pGEX-6P-1 | GST fusion protein expression vector | Amersham |
| pGEX-escC | escC cloned into pGEX-6P-1 | This study |
| pGEX-escJ | escJ cloned into pGEX-6P-1 | This study |
| pDEST15 | GST fusion protein expression vector | Invitrogen |
| p15-escD | escD cloned into pDEST15 | This study |
| p15-escF | escF cloned into pDEST15 | This study |
| pBAD-DEST49 | V5-tagged protein expression vector | Invitrogen |
| p99ccdB-V5 | V5-tagged protein expression vector derived from pTrc99A | This study |
| p99-escD-V5 | escD cloned into p99ccdB-V5 | This study |
| p99-escF-V5 | escF cloned into p99ccdB-V5 | This study |