TABLE 2.
Oligonucleotides used in this studya
| Oligonucleotide | Sequence (5′ to 3′) | Description and position |
|---|---|---|
| abrB-F | CCATCGATCGGCATCTTGAAACCTCCTA | ClaI; 5′ end of PabrB |
| abrB-R | GGAATTCCTTCATAAACATTCTCCTCCC | EcoRI; 3′ end of PabrB |
| spoIIE-F | CCCAAGCTTGGCGGTGTAGCTCAGCTGGC | HindIII; 5′ end of PspoIIE |
| spoIIE-R | TTCTGCAGTTCCATTCCTCTCATCTCCCACCTG | PstI; 3′ end of PspoIIE |
| cotC-F | GGGGTACCCAGAATTTAAACAAGCAACAAGCGG | KpnI; 5′ end of PcotC |
| cotC-R-transl | CCCAAGCTTGTAGTGTTTTTTATGCTTTTTATACTCTAC | HindIII; 3′ end of cotC |
| kinA-F | GCGGATCCGATGACGATGACAAAATGGAACAGGATACGCAGCATGTTAAC | BamHI; 5′ end of kinA |
| kinA-R | GCGCAAGCTTATTTTTTTGGAAATGAAATTTTAAACGC | HindIII; 3′ end of kinA |
| PDG-F | AAGGAGGAAGCAGGTATGAGAGGATCGCATCACCATCACCATCAC | LIC; 5′ end of pQE30-kinA |
| PDG-R | GACACGCACGAGGTTATTTTTTTGGAAATGAAATTTTAAACGCTG | LIC; 3′ end of pQE30-kinA |
a
Relevant restriction sites are underlined. Abbreviations: F, forward; R, reverse; LIC, ligation-independent cloning. Note that the use of the primers for abrB and spoIIE resulted in transcriptional GFP fusions; the primer for cotC resulted in a C-terminal translational GFP fusion under the control of its own promoter; and the primer for kinA resulted in a GFP fusion under the control of an IPTG-inducible promoter.