FIG. 1.

Cellulose production and biofilm formation in E. coli strain 1094. (A) CR- and CF-binding phenotypes of strains 1094, 1094bcsC, 1094csgD, 1094csgA, 1094mlrA, 1094adrA, and 1094rpoS. Two microliters of an o/n culture was spotted onto CR-LB plates (upper row) and CF-LB plates (lower row) and incubated for 48 h at 30°C (CR) or 37°C (CF). (B) Biofilm formation in microfermentors. The biofilms were grown for 24 h at 37°C in M63B1-glu. The biofilm formation ability of the mutant strains is expressed as a percentage of the 1094 wild-type biofilm, set to 100%. The error bars represent standard errors of the means.