FIG. 2.

YedQ contains a GGDEF domain. (A) Sequence alignment of the GGDEF domains of AdrA-Salty and AdrA-1094 from Salmonella and E. coli 1094, respectively, YedQ-1094 from E. coli 1094, and STM1987-Salty (GcpA) from Salmonella. Stars under the sequences identify residues conserved among all the aligned sequences. The GGDEF domain is boxed, and the GGDEF motif is shaded. The two black arrowheads show the positions of the mutated residues in the YedQ_gaaef mutant. The alignment was produced using the program Multalin version 5.4.1 (15). # indicates conservation of a charged amino acid; ! indicates conservation of hydrophobic amino acids. (B) Schematic domain structures of YedQ and AdrA proteins from domain analysis using the Pfam database. Black box, signal sequence; white boxes, potential transmembrane domains; dark-gray boxes, GGDEF domains; light-gray box, MASE2 domain. The size of the protein is indicated in residue numbers; the sizes of the GGDEF and MASE2 domains are indicated under the domains by residue numbers in the protein.