FIG. 3.
YedQ is the regulator for cellulose production in E. coli 1094. (A) CR- and CF-binding phenotypes of strains 1094, 1094yedQ, 1094yedQ(pZE12-yedQ), 1094yedQ(pZE12-yedQgaaef), and 1094yedQ(pZE12-gfp). Two microliters of an o/n culture was spotted onto CR-LB (upper row) and CF-LB (lower row) plates and incubated for 24 h at 30°C. Similar phenotypes were obtained at 37°C and on CF-M63B1 plates. (B) Biofilm formation in microfermentors. The biofilms were grown for 24 h at 37°C in M63B1-glu. The biofilm formation ability of 1094yedQ and complemented mutants is expressed as a percentage of the 1094 wild-type biofilm, set to 100%. The error bars represent standard errors of the means. (C) Complementation of the CR- and CF-binding phenotypes of the Salmonella serovar Enteritidis adrA (3934adrA) mutant by pZE12-yedQ. Two microliters of o/n cultures was spotted onto CR-LB (upper row) and CF-LB (lower row) plates and incubated for 24 h at 30°C.