TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| 1094 | E. coli commensal strain isolated from a healthy male adult; phylogenetic group A as detected by PCR | Gift; C. Le Bouguenec |
| 1094bcsC | ΔbcsC::Km Kmr | This study |
| 1094bcsA-C | ΔbcsA-C::Km Kmr | This study |
| 1094bcsEFG | ΔbcsE-G::Km Kmr | This study |
| 1094csgD | ΔcsgD::aadA Specr | This study |
| 1094adrA | ΔadrA::aadA Specr | This study |
| 1094mlrA | ΔmlrA::aac Aprar | This study |
| 1094yedQ | ΔyedQ::zeo Zeocinr | This study |
| 1094rpoS | ΔrpoS::Km Kmr | This study |
| 1094yedQ-uidA | uidA transcriptional fusion placed under the control of the yedQ promoter activity; Zeocinr | This study |
| 1094csgD-pgi | ΔcsgD::aadA; Specr with a transposon inserted into pgi; Kmr | This study |
| 1125 | E. coli commensal strain | Gift; C. Le Bouguenec |
| 1125bcsC | ΔbcsC::aac Aprar | This study |
| 1125csgD | ΔcsgD::aadA Specr | This study |
| 1125yedQ | ΔyedQ::zeo Zeocinr | This study |
| 1125adrA | ΔadrA::aadA Specr | This study |
| 1125yedQ adrA | ΔadrA::aadA Specr ΔyedQ::zeo Zeocinr | This study |
| DSM6601 | E. coli commensal strain | Laboratory collection |
| DSM6601bcsC | ΔbcsC::Km Kmr | This study |
| DSM6601csgD | ΔcsgD::aadA Specr | This study |
| DSM6601yedQ | ΔyedQ::zeo Zeocinr | This study |
| 55989 | E. coli entero-aggregative pathogenic strain | Gift; C. Le Bouguenec |
| 55989bcsC | ΔbcsC::Km Kmr | This study |
| 55989csgD | ΔcsgD::aadA Specr | This study |
| 55989yedQ | ΔyedQ::zeo Zeocinr | This study |
| 55989adrA | ΔadrA::aadA Specr | This study |
| MG1655 | λ− rph-1 | Laboratory collection |
| MG1655csgD | ΔcsgD::aadA Specr | This study |
| PHL818 | MG1655ompR234 malT::Tn10 Tetr | 43 |
| PHL818csgD | ΔcsgD::aadA Specr | This study |
| 3934 | Salmonella serovar Enteritidis clinical isolate | 58 |
| 3934adrA | ΔadrA::Km | 23 |
| S17-1λpir | RP4-2Tc::mu km::Tn7 λpir; Pir-dependent replication | Laboratory collection |
| EcoR collection | E. coli strains listed in Table S1 in the supplemental material | 41 |
| Plasmids | ||
| pSC189 | Ampr Kmr | 13 |
| pKOBEGA | Arabinose-inducible λ Red recombinase expression plasmid [oriR101 repA101(Ts) ParaB-gam-bet-exo Ampr] | 12 |
| pKOBEG | Arabinose-inducible λ Red recombinase expression plasmid [oriR101 repA101(Ts) ParaB-gam-bet-exo Cmr] | 12 |
| pRS415 | Ampr pMB1 ori; promoterless-lacZ plasmid | 57 |
| pRSpyedQ::lacZ | lacZ fusion placed under the transcriptional control of the yedQ promoter from strain 1094 in pRS415; pMB1 ori | This study |
| pRSpadrA::lacZ | lacZ fusion placed under the transcriptional control of the adrA promoter from strain 1094 in pRS415; pMB1 ori | This study |
| pZE12-gfp | Green fluorescent protein placed under the control of the synthetic lacp promoter on the pZE vector used as a cloning vector; Ampr ColE1 ori | Gift; C. C. Guet |
| pZE12-yedQ | yedQ of strain 1094 placed under the control of the synthetic lacp promoter on the pZE vector; Ampr ColE1 ori | This study |
| pZE12-yedQgaaef | yedQgaaef (mutation in the GGDEF domain) placed under the control of the synthetic lacp promoter on the pZE vector; Ampr ColE1 ori | This study |
| pZE12-adrA | adrA of strain 1094 placed under the control of the synthetic lacp promoter on the pZE vector; Ampr ColE1 ori | This study |
| pZE1Pr-adrA | adrA placed under the control of its own promoter on the pZE vector; Ampr ColE1 ori | This study |
| pSTC14 | rpoS placed under the control of the lacp promoter on the pACYC177 vector; Ampr p15A ori | 40 |