TABLE 2.

Phenotypes and molecular analysis of E. coli 1094csgD CF-binding-deficient mutants

COG group or strain	Insertion sitea	Gene	BLAST description	Phenotypee
				M63B1-glu + CFb		LB + CFb		LB + CRc		Biofilmd
				30°C	37°C	30°C	37°C	30°C	37°C
Information storage/processing	2865100	rpoS	RNA polymerase sigma factor	+/−	+/−	−	−	sap	sap	+
Metabolism	4232307	pgi	Phosphoglucose isomerase	+++	+++	−	−	sar	sar	ND
	1291485	galU	UDP-glucose-1-P uridylyltransferase	−	−	−	−	sar	sar	−
	1290696	galU	UDP-glucose-1-P uridylyltransferase	−	−	−	−	sar	sar	−
Cellular processes	2024925	yedQ	Unknown	−	−	−	−	cdar	cdar	−
E. coli 1094csgD				+++	+++	+++	+++	rdar	rdar	++

^aInsertion site in bp compared to MG1655 sequence.

^bFluorescence under UV light was examined after growth on minimal (M63B1-glu) or rich (LB) agar plates containing CF at 30°C or 37°C for 48 h. The intensity of the fluorescence was estimated visually. The highest fluorescence is expressed as +++; no fluorescence as −.

^cMorphotype on LB-CR plates without NaCl. cdar, circular, dry, and red.

^dBiofilm in microfermentor at 37°C in M63B1-glu medium. The biofilm was grown for 24 hours. Strong biofilm, ++; medium biofilm, +; poor biofilm, −; ND, not determined.

^ePhenotypes compared to 1094csgD levels.