FIG. 3.

Deletion analysis of DNA for relative PmucD expression. A series of lacZ transcriptional fusions were constructed using the lacZ transcriptional fusion vector pSS223 (34) with the 3′ end at +60 bp into the mucD coding sequence and with various amounts of upstream DNA. Plasmids were transferred to PAO1 by conjugation and grown in L broth to an OD₆₀₀ of 1.0, and aliquots were tested for β-galactosidase activity (Miller units). Activity from pLW38a containing the mucB-mucC region upstream of mucD was assigned a value of 100%.