Schematic of the Gli349 molecule. (A) Amino acid sequence. Solid and broken boxes indicate repeats found in Gli349 and positions corresponding to those found only in the ortholog produced by M. pulmonis, MYPU2110, respectively. The names of repeats A to V (13) are indicated on the boxes. The predicted transmembrane segment and epitope of MAb7 are indicated by solid and open triangles, respectively. (B) Amino acid sequence and topology in the cell membrane. The cytoplasm and membrane of a mycoplasmal cell are shown by the meshed area and the thick line, respectively. The glass surface is shown as a gray line. The Gli349 molecule is presented as a rod sticking out of the cell and is supported by other protein components, represented by the gray ellipse. The repeat sequences and gap regions are represented by small ellipses and gray lines, respectively.